Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa.
Identifieur interne : 003578 ( Main/Exploration ); précédent : 003577; suivant : 003579Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa.
Auteurs : M. Schmidt [États-Unis] ; A M Schwartzberg ; P N Perera ; A. Weber-Bargioni ; A. Carroll ; P. Sarkar ; E. Bosneaga ; J J Urban ; J. Song ; M Y Balakshin ; E A Capanema ; M. Auer ; P D Adams ; V L Chiang ; P James SchuckSource :
- Planta [ 1432-2048 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Populus, Végétaux génétiquement modifiés.
- métabolisme : Lignine, Paroi cellulaire, Populus, Végétaux génétiquement modifiés.
- Analyse spectrale Raman.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Lignin.
- cytology : Plants, Genetically Modified, Populus.
- metabolism : Cell Wall, Plants, Genetically Modified, Populus.
- Spectrum Analysis, Raman.
Abstract
Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-microm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm(-1), differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.
DOI: 10.1007/s00425-009-0963-x
PubMed: 19526248
PubMed Central: PMC2715566
Affiliations:
Links toward previous steps (curation, corpus...)
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Schmidt</name><affiliation wicri:level="2"><nlm:affiliation>Energy Biosciences Institute, University of California, Berkeley, CA 94720, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Energy Biosciences Institute, University of California, Berkeley, CA 94720</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Schwartzberg, A M" sort="Schwartzberg, A M" uniqKey="Schwartzberg A" first="A M" last="Schwartzberg">A M Schwartzberg</name></author><author><name sortKey="Perera, P N" sort="Perera, P N" uniqKey="Perera P" first="P N" last="Perera">P N Perera</name></author><author><name sortKey="Weber Bargioni, A" sort="Weber Bargioni, A" uniqKey="Weber Bargioni A" first="A" last="Weber-Bargioni">A. Weber-Bargioni</name></author><author><name sortKey="Carroll, A" sort="Carroll, A" uniqKey="Carroll A" first="A" last="Carroll">A. Carroll</name></author><author><name sortKey="Sarkar, P" sort="Sarkar, P" uniqKey="Sarkar P" first="P" last="Sarkar">P. Sarkar</name></author><author><name sortKey="Bosneaga, E" sort="Bosneaga, E" uniqKey="Bosneaga E" first="E" last="Bosneaga">E. Bosneaga</name></author><author><name sortKey="Urban, J J" sort="Urban, J J" uniqKey="Urban J" first="J J" last="Urban">J J Urban</name></author><author><name sortKey="Song, J" sort="Song, J" uniqKey="Song J" first="J" last="Song">J. Song</name></author><author><name sortKey="Balakshin, M Y" sort="Balakshin, M Y" uniqKey="Balakshin M" first="M Y" last="Balakshin">M Y Balakshin</name></author><author><name sortKey="Capanema, E A" sort="Capanema, E A" uniqKey="Capanema E" first="E A" last="Capanema">E A Capanema</name></author><author><name sortKey="Auer, M" sort="Auer, M" uniqKey="Auer M" first="M" last="Auer">M. Auer</name></author><author><name sortKey="Adams, P D" sort="Adams, P D" uniqKey="Adams P" first="P D" last="Adams">P D Adams</name></author><author><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name></author><author><name sortKey="Schuck, P James" sort="Schuck, P James" uniqKey="Schuck P" first="P James" last="Schuck">P James Schuck</name></author></analytic><series><title level="j">Planta</title><idno type="eISSN">1432-2048</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Lignin (metabolism)</term><term>Plants, Genetically Modified (cytology)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (cytology)</term><term>Populus (metabolism)</term><term>Spectrum Analysis, Raman (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse spectrale Raman (MeSH)</term><term>Lignine (métabolisme)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (cytologie)</term><term>Populus (métabolisme)</term><term>Végétaux génétiquement modifiés (cytologie)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Paroi cellulaire</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Spectrum Analysis, Raman</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse spectrale Raman</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-microm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm(-1), differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19526248</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>230</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>589-97</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-009-0963-x</ELocationID><Abstract><AbstractText>Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-microm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm(-1), differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Energy Biosciences Institute, University of California, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schwartzberg</LastName><ForeName>A M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Perera</LastName><ForeName>P N</ForeName><Initials>PN</Initials></Author><Author ValidYN="Y"><LastName>Weber-Bargioni</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Carroll</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Sarkar</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Bosneaga</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Urban</LastName><ForeName>J J</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Balakshin</LastName><ForeName>M Y</ForeName><Initials>MY</Initials></Author><Author ValidYN="Y"><LastName>Capanema</LastName><ForeName>E A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Auer</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Adams</LastName><ForeName>P D</ForeName><Initials>PD</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>V L</ForeName><Initials>VL</Initials></Author><Author ValidYN="Y"><LastName>Schuck</LastName><ForeName>P James</ForeName><Initials>PJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>06</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013059" MajorTopicYN="N">Spectrum Analysis, Raman</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>05</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19526248</ArticleId><ArticleId IdType="doi">10.1007/s00425-009-0963-x</ArticleId><ArticleId IdType="pmc">PMC2715566</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biopolymers. 2006 Dec 5;83(5):546-55</Citation><ArticleIdList><ArticleId IdType="pubmed">16897765</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2007 Feb 20;17(4):R115-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17307040</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2006 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Auer</name><name sortKey="Balakshin, M Y" sort="Balakshin, M Y" uniqKey="Balakshin M" first="M Y" last="Balakshin">M Y Balakshin</name><name sortKey="Bosneaga, E" sort="Bosneaga, E" uniqKey="Bosneaga E" first="E" last="Bosneaga">E. Bosneaga</name><name sortKey="Capanema, E A" sort="Capanema, E A" uniqKey="Capanema E" first="E A" last="Capanema">E A Capanema</name><name sortKey="Carroll, A" sort="Carroll, A" uniqKey="Carroll A" first="A" last="Carroll">A. Carroll</name><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name><name sortKey="Perera, P N" sort="Perera, P N" uniqKey="Perera P" first="P N" last="Perera">P N Perera</name><name sortKey="Sarkar, P" sort="Sarkar, P" uniqKey="Sarkar P" first="P" last="Sarkar">P. Sarkar</name><name sortKey="Schuck, P James" sort="Schuck, P James" uniqKey="Schuck P" first="P James" last="Schuck">P James Schuck</name><name sortKey="Schwartzberg, A M" sort="Schwartzberg, A M" uniqKey="Schwartzberg A" first="A M" last="Schwartzberg">A M Schwartzberg</name><name sortKey="Song, J" sort="Song, J" uniqKey="Song J" first="J" last="Song">J. Song</name><name sortKey="Urban, J J" sort="Urban, J J" uniqKey="Urban J" first="J J" last="Urban">J J Urban</name><name sortKey="Weber Bargioni, A" sort="Weber Bargioni, A" uniqKey="Weber Bargioni A" first="A" last="Weber-Bargioni">A. Weber-Bargioni</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Schmidt, M" sort="Schmidt, M" uniqKey="Schmidt M" first="M" last="Schmidt">M. Schmidt</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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